Evaluation Of Synergistic Antimicrobial Activity and Antioxidant Activity of blend of Essential Oil contains Fennel, Coriander, Ajowan and Caraway

In the present study evaluation of synergistic antimicrobial activity and antioxidant activity of blend of essential oil contains cardamom, coriander, fennel, caraway and ajowan were carried out. The essential oil was extracted from dried seed of cardamom, Coriander, Fennel, caraway and Ajowan were blend in sunflower oil used as base. The antibacterial activity and minimum inhibitory concentration (MIC) of essential oil against several pathogens was evaluated. The minimum inhibitory concentration (MIC) values of oils were determined by micro broth dilution assay. The results showed that the gram positive and gram negative strains of bacteria had different sensitivities to essential oils. The potential synergetic effect of blend of essential oil with a common antibacterial, Ciprofloxacin, was also studied. Ciprofloxacin and extracted essential oil in combination inhibited the growth of gram positive and gram negative bacteria. Extracted essential oil showed synergistic activity with ciprofloxacin against test strains. Antioxidant activity of the essential oils extracted were carried out using DPPH, H2O2 and Nitric oxide scavenging assay at 100 μg/mL and 200 μg/mL and compared with the standard Ascorbic acid. In the present study, extract blend of essential oil showed a significant effect in inhibiting DPPH, reaching up to 76.84% at concentration of 200 μg/mL. The blend of extract showed a significant effect in inhibiting H2O2, reaching up to 70.04 % at concentration of 200 μg/mL. The extract showed maximum activity of 72.80 % at 200 μg/mL, where as ascorbic acid at the same concentration exhibited 94.96% inhibition in Nitric oxide scavenging assay.